Nazario Olla
Technician
Area of interest:
He’s involved in research activity, vocational training in medical genetics field. He currently employs several Cellular and Molecular Biology methodology. He’s also involved in staff training and he is also in charge for the organizational aspects of the Institute as a logistics manager of the “Shared Services”. He has collaborated in the creation of a biobank of DNA extracted from healthy donors recruited at the Transfusion Center of the Brotzu Hospital.
Most significant publications:
2015
Palomba, Grazia; Loi, Angela; Porcu, Eleonora; Cossu, Antonio; Zara, Ilenia; Budroni, Mario; Dei, Mariano; Lai, Sandra; Mulas, Antonella; Olmeo, Nina; Ionta, Maria Teresa; Atzori, Francesco; Cuccuru, Gianmauro; Pitzalis, Maristella; Zoledziewska, Magdalena; Olla, Nazario; Lovicu, Mario; Pisano, Marina; Abecasis, Gonçalo R; Uda, Manuela; Tanda, Francesco; Michailidou, Kyriaki; Easton, Douglas F; Chanock, Stephen J; Hoover, Robert N; Hunter, David J; Schlessinger, David; Sanna, Serena; Crisponi, Laura; Palmieri, Giuseppe
Genome-wide association study of susceptibility loci for breast cancer in Sardinian population Journal Article
In: BMC cancer, 15 , pp. 383, 2015, ISSN: 1471-2407.
@article{palomba_genome-wide_2015,
title = {Genome-wide association study of susceptibility loci for breast cancer in Sardinian population},
author = {Grazia Palomba and Angela Loi and Eleonora Porcu and Antonio Cossu and Ilenia Zara and Mario Budroni and Mariano Dei and Sandra Lai and Antonella Mulas and Nina Olmeo and Maria Teresa Ionta and Francesco Atzori and Gianmauro Cuccuru and Maristella Pitzalis and Magdalena Zoledziewska and Nazario Olla and Mario Lovicu and Marina Pisano and Gon{ç}alo R Abecasis and Manuela Uda and Francesco Tanda and Kyriaki Michailidou and Douglas F Easton and Stephen J Chanock and Robert N Hoover and David J Hunter and David Schlessinger and Serena Sanna and Laura Crisponi and Giuseppe Palmieri},
doi = {10.1186/s12885-015-1392-9},
issn = {1471-2407},
year = {2015},
date = {2015-05-01},
journal = {BMC cancer},
volume = {15},
pages = {383},
abstract = {BACKGROUND: Despite progress in identifying genes associated with breast cancer, many more risk loci exist. Genome-wide association analyses in genetically-homogeneous populations, such as that of Sardinia (Italy), could represent an additional approach to detect low penetrance alleles.
METHODS: We performed a genome-wide association study comparing 1431 Sardinian patients with non-familial, BRCA1/2-mutation-negative breast cancer to 2171 healthy Sardinian blood donors. DNA was genotyped using GeneChip Human Mapping 500 K Arrays or Genome-Wide Human SNP Arrays 6.0. To increase genomic coverage, genotypes of additional SNPs were imputed using data from HapMap Phase II. After quality control filtering of genotype data, 1367 cases (9 men) and 1658 controls (1156 men) were analyzed on a total of 2,067,645 SNPs.
RESULTS: Overall, 33 genomic regions (67 candidate SNPs) were associated with breast cancer risk at the p < 0(-6) level. Twenty of these regions contained defined genes, including one already associated with breast cancer risk: TOX3. With a lower threshold for preliminary significance to p < 10(-5), we identified 11 additional SNPs in FGFR2, a well-established breast cancer-associated gene. Ten candidate SNPs were selected, excluding those already associated with breast cancer, for technical validation as well as replication in 1668 samples from the same population. Only SNP rs345299, located in intron 1 of VAV3, remained suggestively associated (p-value, 1.16 x 10(-5)), but it did not associate with breast cancer risk in pooled data from two large, mixed-population cohorts.
CONCLUSIONS: This study indicated the role of TOX3 and FGFR2 as breast cancer susceptibility genes in BRCA1/2-wild-type breast cancer patients from Sardinian population.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
METHODS: We performed a genome-wide association study comparing 1431 Sardinian patients with non-familial, BRCA1/2-mutation-negative breast cancer to 2171 healthy Sardinian blood donors. DNA was genotyped using GeneChip Human Mapping 500 K Arrays or Genome-Wide Human SNP Arrays 6.0. To increase genomic coverage, genotypes of additional SNPs were imputed using data from HapMap Phase II. After quality control filtering of genotype data, 1367 cases (9 men) and 1658 controls (1156 men) were analyzed on a total of 2,067,645 SNPs.
RESULTS: Overall, 33 genomic regions (67 candidate SNPs) were associated with breast cancer risk at the p < 0(-6) level. Twenty of these regions contained defined genes, including one already associated with breast cancer risk: TOX3. With a lower threshold for preliminary significance to p < 10(-5), we identified 11 additional SNPs in FGFR2, a well-established breast cancer-associated gene. Ten candidate SNPs were selected, excluding those already associated with breast cancer, for technical validation as well as replication in 1668 samples from the same population. Only SNP rs345299, located in intron 1 of VAV3, remained suggestively associated (p-value, 1.16 x 10(-5)), but it did not associate with breast cancer risk in pooled data from two large, mixed-population cohorts.
CONCLUSIONS: This study indicated the role of TOX3 and FGFR2 as breast cancer susceptibility genes in BRCA1/2-wild-type breast cancer patients from Sardinian population.
- Monserrato
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